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Spheroid invasion assay. Invasion of U87[CD95L] and U251[CD95L] spheroids is shown at the start of the assay and after 72 h. Invasion after CD95L knockdown was induced by the addition of 10 ng/ml APG293 to the assay matrix. Coincubation of APG293 was at room temperature for 30 min with either 100 μg/ml APG101 or 100 μg/ml APG122.
