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. 2015 Jun 24;14:253. doi: 10.1186/s12936-015-0729-9

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© Combrinck et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Haem fractionation profiles for pyrimethamine. Percentages of ‘free’ haem (a) and Hz (b) are shown at increasing concentrations of PYR. No statistical difference exists between the total haem Fe per cell of all PYR treated cells and untreated cells (c). The amount of ‘free’ haem Fe (d) and Hz Fe (e) are shown at increasing concentrations of PYR. PYR IC₅₀ as determined in D10 = 44 ± 6.8 nM