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. Author manuscript; available in PMC: 2015 Sep 20.
Published in final edited form as: J Org Chem. 2015 Mar 5;80(6):3083–3091. doi: 10.1021/jo502948t

Table 2.

Thermal Stability Measurements of 2′,4′-diF-ANA- and 2′-F-ANA-Modified DNA Strands versus Complementary RNA

oligomer modification sequence (5′–3′)a Tm (°C)b ΔTm (°C) ΔTm/mod (°C)
A1 DNA d(GCGTTTTTTGCT) 44.1
A2 2′,4′-diF-araU d(GCGTTUTTTGCT) 44.0 −0.1 −0.1
A3 2′-F-araU d(GCGTTUTTTGCT) 44.7 +0.6 +0.6
B1 DNA d(CUAUAGUAUAC) 25.2
B2 2′,4′-diF-araU d(CUAUAGUAUAC) 25.8 +0.6 +0.15
B3 2′-F-araU d(CUAUAGUAUAC) 26.8 +1.6 +0.4
a

Underlined letter indicates modified residue.

b

Tm values were measured in 10 mM sodium phosphate buffer (pH 7.2) containing 100 mM NaCl and 0.1 mM EDTA. Sequence of RNA complement: 5′-r(AGCAAAAAACGC)-3′ and 5′-r(GUAUACUAUAG)-3′.