Table 1.
Cell density after 24 hours (×104 cells/ml) |
||
---|---|---|
Cell type | Control | 10 ng/ml AprA |
Wild type | 31±2 | 21±2 |
aprA − | 53±4 | 25±3 |
Cells were inoculated at 1×105 cells/ml in shaking culture, and immunoprecipitated AprA or an equal volume of the material immunoprecipitated from aprA− cells (control) was added. The cell density was then determined 24 hours later. Values are means±s.e.m. from three separate experiments. The effect of AprA on wild-type cells is significant (P<0.03) and the effect of AprA on aprA− cells is significant (P<0.01; t-tests).