Table 4.
The effect of AprA on the ability of cells to form spores
| Cell type | Percent of cells forming visible spores | Percent of cells forming detergent-resistant spores |
|---|---|---|
| Wild type | 80±3 | 28±8 |
| aprA − | 42±16 | 5±2 |
| aprA−/actin15∷aprA | 36±12 | 2±1 |
Cells were starved on filter pads, and after fruiting bodies had formed, cells were dissociated and the number of phase-dark spores was counted. The cells were incubated with detergent to kill any vegetative or prestalk cells (a large fraction of spores are resistant to detergent; stalk cells are already dead), and plated with bacteria. The number of colonies was counted 7 days later. The percentage of the original 107 cells forming visible as well as detergent-resistant viable spores was then calculated. Values are mean±s.e.m. from three separate experiments.