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. Author manuscript; available in PMC: 2015 Jun 29.
Published in final edited form as: Cytoskeleton (Hoboken). 2013 Dec 10;71(1):61–78. doi: 10.1002/cm.21159

Fig. 3.

Fig. 3

CLIC5 concentrates at the base of the hair bundle. (A, B) Fluorescence confocal micrographs of adult rat whole mount organ of Corti preparations stained with specific antibody (APB56) against CLIC5 (green); actin filaments were counterstained with phal-loidin (red). Specimens were gently squashed to facilitate visualization of staining along the proximal-distal axis of stereocilia; note that requisite use of heat-induced antigen retrieval compromised the morphological preservation of bundles as well as the quality of F-actin staining. In both inner (A) and outer (B) hair cells, CLIC5 is concentrated at the base of stereocilia (arrows). (C–E) Expression of GFP-CLIC5 fusion protein in vestibular hair cell. The transfected cell (C) exhibits accumulation of CLIC5 towards the proximal end of the stereocilia. (D) Actin filaments were counterstained with phalloidin (red). (E) Merged images of C and D. Scale bars: 5 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]