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. Author manuscript; available in PMC: 2015 Jun 29.
Published in final edited form as: Cytoskeleton (Hoboken). 2013 Dec 10;71(1):61–78. doi: 10.1002/cm.21159

Fig. 9.

Fig. 9

CLIC5 associates with Ezrin and TPRN in LLC-PK1 epithelial cells. (A) Endogenous ezrin coimmunoprecipitates with GFP-CLIC5. Cells transfected with GFP-CLIC5 (GFP-C5) or mock-transfected (Control) were treated with DSP (+) or DMSO alone (−), followed by immunoprecipitation with GFP antibody. Immunoblots of starting lysates (nonreduced and reduced) and immunoprecipitates were probed with ezrin polyclonal antibody and then reprobed with CLIC5 polyclonal antibody. (B) GFP-CLIC5 coimmunoprecipitates with the C-terminal region of TPRN. Cells double transfected with GFP-CLIC5 and Xpress-TPRN308–711 were treated with DSP (+) or DMSO alone (−), followed by immunoprecipitation with antibody against the Xpress epitope (X) or an isotype matched control (C) monoclonal antibody. Immunoblots were probed with CLIC5 polyclonal antibody and then reprobed with TPRN polyclonal antibody (TPRN); band indicated by the arrow corresponds to CLIC5. (C) Xpress-TPRN308–711 coimmunoprecipitates with GFP-CLIC5. Double transfected cells were treated with DSP (+) or DMSO (−) followed by immunoprecipitation with antibodies against GFP (G) or a control (C) polyclonal antibody. Immunoblots were probed with TPRN polyclonal antibody and then reprobed with whole antiserum against CLIC5. IgG heavy chain (Ig HC) is indicated.