Fig. 4. mTOR signaling regulates the expression of key myogenic genes.
(A, B) Relative levels of mTOR, Pax7, MyoD and MyoG proteins (A) and mRNA (B) in myoblasts depleted of Mtor (4-OH-TMX) and control myoblasts (vehicle treated). N=4. (C, D) Relative levels of phosphorylated S6 (pS6), total S6, Pax7, MyoD and MyoG proteins (C) and mRNA (D) in WT myoblasts treated with rapamycin (RAPA) and vehicle control. N=4. * p < 0.05, ** p < 0.01.