Skip to main content
. 2015 Jun 17;4(6):1293–1311. doi: 10.3390/jcm4061293

Figure 3.

Figure 3

Figure 3

Distribution of neuropilin-1 protein in kidney glomeruli of diabetic db/db mice and non-diabetic db/m littermates. Double immunological detection of neuropilin-1 (NRP-1) and synaptopodin (SYN), a podocyte specific marker protein. Nuclei were counterstained with DAPI. Images were analyzed using LSM 510 META and ZEN 2009 software (Zeiss, Germany). The staining was performed on 2 µm paraffin kidney sections originated from diabetic db/db mice and non-diabetic db/m littermates. The co-localization of the NRP-1 and synaptopodin proteins is presented as a merge image. Bars correspond to 20 µm. Magnification 400x. (A) Protein expression in glomeruli of non-diabetic db/m mice. (B) Protein expression in glomeruli of diabetic db/db mice. The expression of NRP-1 is reduced in db/db mice (B) compared with non-diabetic db/m littermates (A). The NRP-1 stain is co-localized with synaptopodin, as seen on the merged images.