Skip to main content
. 2015 Jul;146(1):65–77. doi: 10.1085/jgp.201411336

Figure 3.

Figure 3.

MgATP reactivates hTRPM3 through PI4Ks. Excised inside-out patch measurements have been performed on TRPM3-expressing Xenopus oocytes with 100 µM PregS in the patch pipette as described in Materials and methods; data are plotted at 100 and −100 mV. (A and B) Representative traces for the effects of MgATP in the absence and presence of LY294002 (LY); the applications of 2 mM MgATP, 300 µM LY294002, and 25 µM diC8 PI(4,5)P2 are indicated by the horizontal lines. (C) Summary of the data for control and 10 and 100 µM LY294002 (n = 5 for control and 300 µM LY and n = 3 for 10 nM LY). (D and E) Representative traces for the effects of MgATP in the absence and presence of the PI4K inhibitor A1; the applications of 2 mM MgATP 100 nM A1 and 25 µM diC8 PI(4,5)P2 are indicated by the horizontal lines. (F) Summary of the data for control and 10 and 100 nM A1 (n = 5–6). (G) Chemical formula for compound A1. Error bars represent SEM. **, P < 0.01; ***, P < 0.005.