Figure 7.

The effects of the rapamycin-inducible 4′- and 5′-phosphatase pseudojanin. (A) Representative measurement in a HEK cell expressing the mTRPM3 and the components of the rapamycin-inducible 4′- and 5′-phosphatase pseudojanin. Measurements were performed using a ramp protocol from −100 to 100 mV, and current amplitudes are plotted at 100 and −100 mV. The applications of 25 µM PregS and 100 nM rapamycin are indicated by the horizontal lines. (B) Similar measurements as in A in a cell transfected with a pseudojanin construct in which the 4′-phosphatase was inactivated. (C) Similar measurements as in A in a cell in which both the 5′- and the 4′-phosphatase domains in pseudojanin were inactivated. (D) Summary of the extent of inhibition at the end of rapamycin application. (E) Time course of inhibition for the three pseudojanin constructs and for the 5′-phosphatase and its control construct from Fig. 6. Error bars represent SEM. *, P < 0.05; **, P < 0.01.