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. 2015 Jun 8;112(25):7821–7826. doi: 10.1073/pnas.1509744112

Fig. S2.

Fig. S2.

Quantitative immunocytochemistry of hUPF1-EGFP in transfected neurons. (A) Primary rodent cortical neurons were transfected with hUPF1-EGFP, fixed and probed using antibodies that recognize total UPF1. The amount of UPF1 in transfected neurons (arrow) was compared with endogenous UPF1 in untransfected neurons (arrowhead) to calculate the fold overexpression. (Scale bar, 50 µm.) (B) Primary neurons cotransfected with hUPF1-EGFP and WT and mutant versions of TDP43 or FUS demonstrated a 3-fold increase in UPF1 antibody reactivity by quantitative ICC. n = 316–433 neurons per genotype. (C) The relationship between hUPF1-EGFP intensity and total UPF1 levels was determined for individual neurons using linear regression analysis. Dotted lines, 95% confidence intervals. n = 76 neurons. Data were pooled from eight separate wells.