Skip to main content
. 2015 Jun 8;112(25):7821–7826. doi: 10.1073/pnas.1509744112

Fig. S5.

Fig. S5.

Evaluation of NMD inhibitor 1 (NMDI) and mutant hUPF1(R844C) in primary neurons. (A) Primary cortical neurons were treated with NMDI or vehicle (DMSO) at 5 µM, transfected with EGFP, and followed by LFM. Vehicle, n = 170 cells; NMDI, n = 132 cells. ns, not significant (P > 0.05), Cox proportional hazards analysis. Results were combined from eight wells per condition in duplicate. (B) Expression of hUPF1(R844C) in transfected neurons (arrow) was confirmed by immunocytochemistry using antibodies that recognize endogenous (arrowheads) as well as exogenous UPF1. (Scale bar, 25 µm.) (C) Fold overexpression of hUPF1(WT) or hUPF1(R844C) was determined by comparison of antibody reactivity in transfected (TF) vs. nontransfected (NT) neurons. hUPF1(WT), n = 197 neurons each for NT and TF groups; hUPF1(R844C), n = 144 neurons each for NT and TF groups. Error bars, ± SEM. **P < 0.0001 vs NT by 2-tailed t test. Data were pooled from four experiments.