Comparison of neutralizing potency between Igs with different quaternary structures. (A) Neutralizing potency of various-sized antibodies against Victoria virus was defined as the MNC of antibodies (IgM + IgG + IgA) in each fraction. (B) Proportions of IgM, IgG, and IgA quantity (wt/wt) in each quaternary structure to total antibody quantity in each quaternary structure (IgM + IgG + IgA, weight). The mean MNC of each quaternary structure of antibody against Victoria virus (C) and NY virus (D) is shown. (E) Mean MNC of each quaternary structure of antibody against Indonesia virus (H5N1) from subjects vaccinated with H5N1 vaccines. (F) Proportion of IgA quantity (wt/wt) in each quaternary structure to total nasal IgA (weight). (G) Binding activities of IgA with various quaternary structures specific for HA of Victoria virus were defined as the MBC of IgA in each fraction. The mean MBC of each quaternary structure of IgA specific for HA of Victoria virus (H) and NY virus (I) is shown. All error bars are SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Poly, polymer; Tetra, tetramer; Tri, trimer.