Fig. 2.

CYP4F22 is involved in ω-hydroxyceramide synthesis. HEK 293T cells were transfected with plasmids encoding 3xFLAG-ELOVL4, 3xFLAG-CERS3, and 3xFLAG-CYP4F subfamily members, as indicated. (A) Total lysates prepared from the transfected cells were separated by SDS/PAGE, followed by immunoblotting with anti-FLAG antibodies. (B) Cells were labeled with [³H]sphingosine for 4 h at 37 °C. Lipids were extracted, separated by normal-phase TLC, and detected by autoradiography. Cer, ceramide; GlcCer, glucosylceramide; SM, sphingomyelin; SPH, sphingosine; ω-OH, ω-hydroxy.