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. 2015 Jun 8;112(25):7707–7712. doi: 10.1073/pnas.1503491112

Fig. S5.

Fig. S5.

MRM chromatogram of ω-hydroxyceramide species produced by CYP4F22. Lipids were extracted from HEK 293T transfected with pCE-puro 3xFLAG-ELOVL4 and pCE-puro 3xFLAG-CERS3, together with control vector (A) or pCE-puro 3xFLAG-CYP4F22 (B). EOS from mouse epidermis was treated with an alkali to liberate ω-hydroxyceramides (C). Lipids were subjected to UPLC/ESI-MS. Each ceramide was detected by MRM by setting the appropriate [M+H]+ and [M+H−H2O]+ values at Q1 and m/z 264.2 at Q3. Each MRM peak was overlaid using MassLynx software. ωhC30:1, ω-hydroxyceramide with a chain length of C30:1. IS, internal control (C17:0 ceramide). The Inset in C shows an enlarged large view of the indicated area of the original chromatogram.