Pederin |
>80% identity to sequences from P. aeruginosa. The discovered ped gene cluster is believed to be from a symbiont of the Paederus beetle from the genus Pseudomonas
|
Targeted sequencing-based strategy |
Paederus beetles |
Piel, 2002
|
Biotin |
Highest identity to proteins from Erwinia herbicola. Significant identity also shown to proteins from E. coli and Pseudomonas putida
|
Selelction-based screening of enriched cosmid library in E. coli biotin auxotrophic strain |
Horse excrement |
Entcheva et al., 2001
|
Known siderophore: vibrioferrin |
98% identity to proteins from Vibrio parahaemolyticus and Vibrio alginolyticus
|
Function-based screening of E. coli plasmid library |
Tidal-flat sediment, Ariake Sea |
Fujita et al., 2011
|
Polyketide synthase (PKS) gene |
55–59% identity to hypothetical PKS from Mycobacterium avium (NP_961164) |
Targeted sequencing-based strategy |
Marine sponge Discodermia dissoluta, Netherlands Antilles |
Schirmer et al., 2005
|
Novel serine protease inhibitor (serpin) gene |
Moderate identities to serpins from Salinibacter ruber M8 and Spirosoma linguale DSM 74. Similarities with possible partial serpins from Dyadobacter fermentans DSM 18053, Arthrospira maxima CS-328 and Cyanothece sp. PCC 7822 |
Sequence-based screening of E. coli plasmid library. |
Uncultured marine organisms |
Jiang et al., 2011
|
Borregomycin A and B encoded by bor pathway (antiproliferative and antibiotic properties) |
ORFs showing 32–86% identity to species from the following genera: Micromonospora, Streptomyces, Actinoplanes, Corallococcus, Cellulomonas, Actinomadura, Salinispora, Microlunatus, Modestobacter, Frankia, Saccharomonospora, Nocardia, Phaeosphaeria
|
Homology guided screening |
Soil, Anza-Borrego Desert (CA) |
Chang and Brady, 2013
|
Hypothetical protein with NF-kB pathway stimulatory activity |
42% of predicted genes coverage to B. vulgatus ATCC 8482 |
Activity-based screening using a reporter cell line of an E. coli fosmid library |
Human gut microbiota of Crohn's Disease patients |
Lakhdari et al., 2010
|
Novel prebiotic degradation pathways (11 contigs) |
Sequence homology to species of Bifidobacterium, Eubacterium, Streptococcus, Bacteroides, Faecalibacterium
|
Hydrolytic activity-based selective screening of two E. coli fosmid libraries |
Human ileum mucosa and fecal microbiota samples |
Cecchini et al., 2013
|
Five novel putative salt tolerance genes |
Identity to hypothetical proteins from genus Collinsella, Eggerthella, and Akkermansia
|
Function-based screening of E. coli plasmid library |
Human gut microbiota |
Culligan et al., 2012
|
Novel salt tolerance gene |
Not homologous to any sequence at time of study, highest BLAST score to hypothetical protein from Caulobacter crescentus
|
Function-based screening of E. coli plasmid library |
Faecal sample, healthy 26 year old Caucasian male |
Culligan et al., 2013
|
15 acid resistance genes |
37–90% identity to proteins and hypothetical proteins from the following genera: Thermosinus, Streptomyces, Candidatus, Hyphomicrobium, Methylococcus, Acidithiobacillus, Thioalkalivibrio, Nitrosococcus, Halorhodospira, Haliangium, Clostridium, Roseomonas, Acidiphilium, Gemmata, Terriglobus, Burkholderia
|
Function-based screening of six E. coli plasmid libraries. Followed by expression in Pseudomonas putida and Bacillus subtilis
|
Planktonic and rhizosphere microbial communities of the Tinto River. Five libraries from Erica andevalensis, one from headwaters of Tinto River |
Guazzaroni et al., 2013
|