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. 2015 Jun 30;6:105. doi: 10.3389/fendo.2015.00105

Figure 2.

Figure 2

Outline of phenotyping protocol for EUCOMM mice used by the Sanger Institute Mouse Genetics Project. The Sanger Institute Mouse Genetics Project describes a standardized series of tests applied to novel EUCOMM lines. Chimeric mice are outcrossed with C57BL/6 to determine germline transmission (GLT) and heterozygous tm1a mice intercrossed to assess viability at 14 days after birth. If viable, homozygous tm1a mice enter the Mouse Genetics Project (MGP) standardized phenotyping pipeline or are tested for fertility. In the case of embryonic lethality or poor survival, heterozygous mice are used for the MGP pipeline and homozygous embryos examined at E14.5 to determine the cause of sub-viability. In parallel to the intercross protocol, a subset of heterozygous tm1a mice are crossed to Cre recombinase mice to generate lacZ reporters. Figure adapted from White et al. (22).