Table 1.
Germline transmission of genes of interest using EUCOMM ES cells.
| Clone | Karyotype | Injected | # injections | # chimeras | Chimeras mated |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Injection # | % | # litters | # pups | # hets | # litters for hets | |||||
| GOI-1#1 | 15/20 40, Y | Yes | 3 | 21 (5F) | 1 | M 90 | 5 | 29 | 0 | – |
| 1 | M 75 | 4 | 63 | 0 | – | |||||
| 1 | M 60 | 0 | 0 | – | – | |||||
| 1 | M 55 | 7 | 63 | 0 | – | |||||
| 1 | M 50 | 8 | 74 | 0 | – | |||||
| 3 | M 95 | 0 | 0 | – | – | |||||
| 3 | M 80 | 8 | 68 | 5 | 1 | |||||
| 3 | M 65 | 8 | 56 | 0 | – | |||||
| GOI-1#2 | Abnormal karyotype | No | – | – | – | – | – | – | – | – |
| GOI-1#3 | Abnormal karyotype | No | – | – | – | – | – | – | – | – |
| GOI-1#4 | 16/20 40, Y | No – not targeted | – | – | – | – | – | – | – | – |
| GOI-2#1 | 10/20 40, Y | No, low karyotype | – | – | – | – | – | – | – | – |
| GOI-2#2 | Clone unavailable | – | – | – | – | – | – | – | – | – |
| GOI-2#3 | 15/20 40, Y | Yes | 2 | 0 | – | – | – | – | – | – |
| GOI-2#4 | 13/20 40, Y | Yes | 4a | 4 | 2 | M 65 | 0 | – | – | – |
| 3 | M 70 | 5 | 49 | 21 | 1 | |||||
| 3 | M 60 | 1 | 12 | 0 | – | |||||
| 4 | M 60 | 2 | 17 | 0 | – | |||||
Hets, heterozygotes.
aNote that only three injections were required for germline transmission. The fourth injection was performed in parallel as a back-up.