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. 2015 Apr;94(4):608–614. doi: 10.1177/0022034515572441

Figure 4.

Figure 4.

Transmission electron microscopy images of mineral and corresponding selected area electron diffraction (SAED) pattern. (A) Representative mineral crystal nucleated by the pB peptide; (B) corresponding SAED diffraction pattern for mineral crystal nucleated by pB that displays a diffuse (2 1 1) ring that is characteristic of impure hydroxyapatite (HA); (C) representative mineral crystal nucleated by a 1:4 ratio of pA:pB; (D) SAED diffraction pattern corresponding to mineral nucleated by a 1:4 ratio of peptides, resulting in a (0 0 2) ring as well as several concentric rings, indicating the presence of rod-like HA crystals. (E, F, G) TEM images obtained from dentin wafers coated with 1:4 peptide, DMP1, and BSA, respectively. (H, I, J) Corresponding magnified images. Intrafibrillar and interfibrillar mineral deposition is clearly observed in (H) and (I). Scale bars for E–G are 1 µm, and those for E′–G′ are 200 nm. The white arrows point to nucleated HAP, and red arrows are tissue folds. This figure is available in color online at http://jdr.sagepub.com.