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. 2015 Jun 30;5:11737. doi: 10.1038/srep11737

Figure 2. Enzymatic measurement of PG + CL.

Figure 2

(a) and (b) Standard curves for PG + CL measurement. The heart CL standard solution was added to Reagent L1 and incubated at 37 °C for 30 min. Then, Reagent L2 was added. After 30 min of incubation at room temperature, Stop Reagent was added. The fluorescence intensity was measured using a microplate reader. Background fluorescence was 3973 ± 57, which was subtracted from each value. Each point represents the mean ± S.D. of triplicate measurement. The lines were obtained by linear regression analysis. The correlation coefficients were r = 0.9996 (a) and r = 0.9998 (b). (c) Fluorescence changes in response to heart CL, TOCL, egg PG, soy PG, POPG and LPG in PG + CL measurement. Each bar represents the mean ± S.D. of triplicate measurement. There were no statistically significant differences between heart CL, TOCL, egg PG, soy PG, POPG and LPG. (d) Linearity of PG + CL measurement. The lipid extract from HEK293 cells was sequentially diluted with 1% Triton X-100. The correlation coefficient was r = 0.994.