Skip to main content
. 2015 Jun 30;5:11788. doi: 10.1038/srep11788

Figure 2. Results of five treatment protocols to clear Bsal infections from experimentally infected fire salamanders.

Figure 2

After ascertaining presence of Bsal in all animals (day 0) they were either left untreated as control (A), treated twice a day for 10 days with voriconazole sprays (12.5 μg/ml) (B) or itraconazole sprays (0.6 μg/ml) (D) alone or with a combination of polymyxin E submersion baths (2000 IU/ml, 10 minutes) followed by spraying voriconazole (12.5 μg/ml) (C) or itraconazole (0.6 μg/ml) (E). Conditions A-E were all performed at an ambient temperature of 15 °C. An additional treatment protocol composed of polymyxin E submersion baths (2000 IU/ml, 10 minutes) followed by spraying voriconazole (12.5 μg/ml) at an ambient temperature of 20 °C was instituted after failure to clear Bsal infections in conditions A-E. Swabs for Bsal real-time PCR analysis were collected at day 0, 10, 17, 24 and 31. Animals that reached the determined experimental endpoint. Each symbol represents the course of infection of an individual animal.