Steps	Temp.	Time	No. of cycle
Reverse transcriptase	50°C	30min	1
Initial PCR activation step	95°C	1min	1
Denaturation	95°C	10s	34
Annealing	50°C	10s	34
Extension	72°C	30s	34
Final extension	72°C	10min	1
Hold	4°C