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. 2015 Jun 30;9:166. doi: 10.3389/fnbeh.2015.00166

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Copyright © 2015 Mamad, McNamara, Reilly and Tsanov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ChR2-YFP expression in the medial septum of ChAT::Cre rats. (A) Atlas schematic of our experimental setup investigating the functional relation of septal activity to the hippocampal formation (MS, medial septum; HIP, hippocampus). The medial septum of ChAT::Cre rats was injected with cre-inducible ChR2-EYFP. Chronically-implanted headstage with optic fiber and microdrive allowed parallel application of blue laser light and measurement of single unit activity in medial septum. Additionally, the implantation of bipolar concentric electrode allowed electric stimulation. Concurrently, recording tetrodes were implanted in hippocampal area CA1 to measure neuronal and local field activity. Coronal atlas schematic (below) and histological section (right) show where optic fiber and eight tetrodes were implanted and subsequently lowered in medial septum. The black arrow indicates the location of tetrodes tip. (B) Colocalization of ChAT staining and ChR2-YFP expression in the medial septum. High-magnification views of ChR2-YFP expression in ChAT-positive septal cell bodies after injection of cre-dependent virus in the medial septum of ChAT::Cre rats.