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. 2015 Jun 30;5:11781. doi: 10.1038/srep11781

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Copyright © 2015, Macmillan Publishers Limited

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(a) Western blot analysis on two independent samples of tumor homogenates for levels of ATF4 in mixed background tumors. β-tubulin was used as a loading control. The ratio of ATF4 to β-tubulin is displayed below each blot. (b) Graphical representation of the ATF4 to β-tubulin ratios calculated from the blots in (a). Data are represented as the average value for each genotype ± standard deviation. Results are not statistically significant. (c) qPCR analysis of ASNS levels in GCN2^+/+ and GCN2^−/− sarcomas. ASNS levels were normalized to the geometric mean of the reference genes β-tubulin, β-actin, and 18S rRNA. Data are represented as the average value for each genotype ± standard deviation. Results are not statistically significant. (d) qPCR analysis of ATF3 levels in GCN2^+/+ and GCN2^−/− sarcomas. ATF3 levels were normalized to the geometric mean of the reference genes β-tubulin, β-actin, and 18S rRNA. Data are represented as the average value for each genotype ± standard deviation. Results are not statistically significant. (e) qPCR analysis of ULK1 levels in GCN2^+/+ and GCN2^−/− sarcomas. ULK1 levels were normalized to the geometric mean of the reference genes β-tubulin, β-actin, and 18S rRNA. Data are represented as the average value for each genotype ± standard deviation. Results are not statistically significant.