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. 2015 Jun 30;5:11777. doi: 10.1038/srep11777

Figure 1. NPMc is phosphorylated earlier in the cell cycle than wtNPM.

Figure 1

HEK293T stably expressing GFP, GFP-wtNPM (wtNPM) or GFP-NPMc (NPMc) were stained with rabbit anti-phosphoNPM(T199) followed by anti-rabbit Alexa fluor 568 conjugate. Unlike wtNPM and GFP expressing cells, immunofluorescence staining shows that some cells have phosphorylation of NPMc in the cytoplasm before nuclear membrane dissolution resulting in a “doughnut” shaped staining pattern around the nucleus (arrowhead). Bar = 20 μM.