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. 2015 Jun 30;5:11777. doi: 10.1038/srep11777

Figure 2. Cdk2-dependant NPMpT199 is hyperphosphorylated in NPMc and unique to pre-mitotic phosphorylation sites.

Figure 2

(a) Western blot shows that knockdown of cdk2 reduces T199 phosphorylation and that NPMc phosphorylation is elevated compared to wtNPM. (b) Pre-mitotic phosphorylation site serine 4 (NPMpS4) also shows similar disparity between wtNPM and NPMc but there is no such difference for other centrosome related sites serine 125 (NPMpS125) or threonines 234 and 237 (NPMpT234/7). G, HEK293T stably overexpressing GFP; W, HEK293T stably overexpressing GFP-wtNPM; C, HEK293T stably overexpressing GFP-NPMc.