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. 2015 Jun 30;5:11777. doi: 10.1038/srep11777

Figure 5. Alanine-mutated NPMc mimics G2/M phase arrest caused by cdk2 inhibition.

Figure 5

(a) Light microscope images of HEK293T transfected with GFP-NPMc (NPMc) or GFP-NPMc with mutated T199A (T199A). Both (NPMc) (T199A) have the same subcellular localization (cytoplasm). Bar = 100 μM. (b) Western blot shows that NPMcT199A is not detectable by antibody specific for phosphorylated NPM (NPMpT199). (c) HEK293T were transfected with GFP-NPMc (NPMc) or GFP-NPMc with mutated T199A (T199A) and treated with GW8510 or the solvent DMSO as per Fig. 3 24 hours post-transfection. Results show mean and standard error from three independent experiments. *p-value < 0.05, **p-value < 0.01. Statistical significance was calculated using student’s T-test compared to the respective NPMc treatment. G0/G1, cells with 2n DNA content; S, cells synthesizing DNA; G2/M, cells with 4n DNA content.