Table 1.
Primer and probes for real time reverse transcription polymerase chain reaction (RT-PCR).
| Primer/Probe | Position | Sequence 5′–3′ | Reference |
|---|---|---|---|
| Rift Valley fever virus (RVFV)-L-forward | 2912–2933 | TGA-AAA-TTC-CTG-AGA-CAC-ATG-G | Bird et al. (2007) |
| RVFV-L-reverse | 2981–3001 | ACT-TCC-TTG-CAT-CAT-CTG-ATG | |
| RVFV-L-probe | 2950–2977 | (CAL Red)a CAA-TGT-AAG-GGG-CCT-GTG-TGG-ACT-TGT-G (Iowa Black)b | |
| MP12-L-forward | 3296–3316 | CCT-CAC-TAT-TAC-ACA-CCA-TTC | Wilson et al. (2013) |
| MP12-L-reverse | 3436–3453 | ATC-ATC-AGC-TGG-GAA-GCT | |
| MP12-L-probe | 3371–3385 | (FAM)a CTG-AGA-TGA-GCA-AGA (Iowa Black)b | |
| Beta actin-forward | BTC-CTT-CCT-GGG-CAT-GGA | Moniwa et al. (2007) | |
| Beta actin-reverse | GRG-GSG-CGA-TGA-TCT-TGA-T | ||
| Beta actin-probe | (CY5)a TCC-ATC-ATG-AAG-TGY-GAC-GTS-GAC-A (Iowa Black)b |
aFluorescent reporter dye; bQuencher.