Figure 5.

Relative intracellular amounts of the antisense RNA variants. Oligo-RNA band-shifts assays were performed with total RNA extracted from pneumococcal cells containing the different pCGA9 recombinant plasmid variants. Arrows point to the position of the free oligonucleotide (lower band) and that of the oligo-RNAII hybrids (upper band). B, sample lacking any RNA; -, sample containing in vitro synthesized mRNA₈₀ and lacking antisense RNA;. Lane 1, samples containing 4 μg total RNA from cells that encode the indicated antisense RNA. Samples prepared with two independent extractions of RNAII wt are shown. Lanes 2 and 3, samples containing 0.025 and 0.0025 pmol, respectively, of the indicated in vitro synthesized antisense RNAs. The table below shows the amount of antisense RNA (pmol) per μg of total RNA. The values displayed are the mean of at least four independent assays.