Table 1.

List of plasmids used in this work.

Plasmid	Size (bp)	Marker	Characteristics	References
pLS1	4408	TcR	Δmob, ΔssoU derivative of pMV158	Lacks et al., 1986
pC194	2907	CmR	Copy-up mutant of pC194 (~110 copies per chromosome equivalent in S. pneumoniae)	Ballester et al., 1990
pCGA9	3070	CmR	rnaII gene of pMV158 cloned in pC194	del Solar et al., 1995
pCGA9-m1	3070	CmR	rnaII-m1 mutant gene cloned in pC194	This work
pCGA9-m2	3070	CmR	rnaII-m2 mutant gene cloned in pC194	This work
pCGA9-m3	3070	CmR	rnaII-m3 mutant gene cloned in pC194	This work
pCGA9-m4	3070	CmR	rnaII-m4 mutant gene cloned in pC194	This work
pALTER-T7-copG-repB-cat	7545	CmR	Recombinant plasmid that carries copG, repB and cat genes of pJS3 cloned in pALTER-1 under the control of the ϕ10 promoter of phage T 7	del Solar et al., 1997