Table 2.
Efficiency of binding of the different antisense RNA variants to mRNA80.
| mRNA80 | |||||
|---|---|---|---|---|---|
| Antisense RNA | Binding rate constant (ka) (105 M−1s−1) | Relative ka | 1/K*d (nM−1) | Bmax ± s.d. (%) | Relative 1/Kd |
| wt | 3.75 ± 0.50 | 1 | 0.56 ± 0.05 | 102.39 ± 0.68 | 1 |
| m1 | 1.08 ± 0.11 | 0.29 | 0.17 ± 0.01 | 103.56 ± 0.64 | 0.30 |
| m2 | 0.02 ± 0.001 | 0.005 | 0.013 ± 0.001 | 67.58 ± 1.46 | 0.023 |
| m3 | 0.66 ± 0.09 | 0.18 | 0.10 ± 0.01 | 106.26 ± 1.19 | 0.18 |
| m4 | 2.29 ± 0.06 | 0.61 | 0.43 ± 0.02 | 103.89 ± 0.62 | 0.77 |
Values are the mean of at least three independent assays.
*
Kd, concentration of free mRNA for half-maximal binding.