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Hematoxylin and eosin (H&E) staining. a, d, g, and j show the H&E staining structure of the disc at 0, 4, 8 and 12 weeks, respectively. b-c show the H&E staining structure of normal discs of different magnification (b 100×, c 200×). d-l show the H&E staining structure of the discs exhibiting a gradual degeneration at different time points. Black arrows show the misalignment of the annulus fibrosus (AF) magnified 16× in images d, g, and j, 100× in e and h, and 200× in f, i, and l
