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. 2015 Jun 20;22(18):1633–1645. doi: 10.1089/ars.2014.6078

FIG. 4.

FIG. 4.

IsoLG-PE activates NFκB to induce TNFα in RAW264.7 cells. (A) Effects of IsoLG-PE and PE on NFκB reporter activity in transfected RAW264.7 murine macrophages. Results are shown as mean±SEM, n=4 replicates and normalized to expression in vehicle-treated cells (0 nM), Two-way ANOVA, p<0.0001 for concentration, treatment, and interaction, *p<0.05 for IsoLG-PE versus PE, Bonferroni's multiple-comparisons test for the same concentration of each phospholipid. (B) PS-1145 inhibition of NFκB activator IκKβ blocks IsoLG-PE induction of TNFα protein expression. Results are shown as mean±SEM of all replicates from experiments on 2 separate days, n=3–4 replicates per day, and normalized to expression in cells treated with vehicle (0 nM) on that day. Two-way ANOVA, p<0.0001, concentration and treatment. *p<0.01 Bonferroni's multiple-comparisons test for vehicle versus PS-1145 for the same concentration of IsoLG-PE. IκKβ, Iκ kinase β; NFκB, nuclear factor kappa B.