FIG. 2.

Representative plots of FACS analyses of TJPs on H9-NSCs. (A) Demonstrates typical gating parameters for subsequent study of H9-NSC populations. Analyses were based upon 10,000 cell counts within the gate. (B) H9-NSCs were immunostained for Nestin and Sox2 to confirm NSC phenotype. Sox2 expressing H9-NSCs were colabeled for TJPs ZO-1 (C), occludin (D), claudin-1 (E), claudin-3 (F), and claudin-5 (G). Number in the quadrants of each plot indicates the percentage of cells showing no expression of the target (bottom left quadrant), Sox2 only (upper left quadrant), the specific TJPs (bottom right quadrant), or both Sox2 and the particular TJP (upper right quadrant).