FIG. 4.

Distribution of TJPs on F-NSCs. (A and B) Expanded cultures of F-NSCs neurospheres show immunopositive reactivity with antibodies to stem cell markers Nestin and Sox2, respectably. (D, G, J, M, and P) Nestin-positive neurospheres also appear immunopositive for TJPs: ZO-1 (E), occludin (H), claudin-1 (K), claudin-3 (N), and claudin-5 (Q). Note the transmembrane and/or cytosolic localization of TJPs, while Nestin appears cytosolic and filamentous. The images on the right column (C, F, I, L, O, and R) show the preceding two images merged along with the nuclear counterstain DAPI. All images are shown at 40× objective power with scale bars at 20 μm. Arrows indicate areas where the expression appears intercellular.