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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 8;95(3):365–382. doi: 10.1111/mmi.12855

Figure 6. Localization of CT009 or MreB within C. trachomatis.

Figure 6

L929 cells infected with C. trachomatis L2 were fixed at 16 hpi and stained with affinity-purified antibodies and images acquired by confocal microscopy with 150× objective. Two or three representative inclusions are shown for

A. Anti-MreB (green), anti-OmpA (red), and merged image

B. Anti-CT009 (green), anti-OmpA (red), and merged image White arrows are highlighting potential sites for bacterial cell division.

C. Anti-OmpA (red), anti-CT009 (green), anti-FLAG (blue), and merged image of CT009 and FLAG

D. Monospecificity of CT009 and MreB antibodies. Expression levels of CT009, MreB and RpoA from C. trachomatis L2 434/Bu were analyzed at 20 hpi. Western blot was performed with affinity purified rabbit anti-CT009, rabbit anti-MreB, or rat anti-RpoA. Calculated MW of each protein are as follows: CT009, 16.2 kDa; MreB, 39.5 kDa; and RpoA, 41.8 kDa.