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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Lab Chip. 2015 May 22;15(13):2781–2789. doi: 10.1039/c5lc00503e

Fig. 4. Attractive and repulsive netrin receptors localize to the tip of neocortical axons extending in a netrin-1 gradient.

Fig. 4

Schematic showing where each µgroove sits within the netrin-1 gradient. Images of neo-cortical axons within the axon viewing area from the indicated µgroove. Axons were stained with phalloidin (blue), an antibody against DCC (green), and an antibody against UNC5H (red). Both DCC and UNC5H localized to the growth cones and axon tips of growth cones in the low netrin concentrations/attractive regime (µgroove 6–10), and the higher netrin concentrations, repulsive regime (µgroove 1–3). Graphs show quantification of surface levels of the netrin receptors in growth cones extending from indicating µgrooves at indicated times in a netrin-1 gradient (starting concentration 600 ng ml−1).