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. Author manuscript; available in PMC: 2015 Jun 30.
Published in final edited form as: J Immunol. 2012 Oct 26;189(11):5411–5420. doi: 10.4049/jimmunol.1201487

Figure 7. OA-NO2 Inhibits LPS-induced Neutrophil Transmigration in Vitro via an ePPARγ–dependent Mechanism.

Figure 7

MLEC were isolated from ePPARγ+/+ and ePPARγ−/− mice (n = 7–8 mice per group) and then grown to confluent monolayers in Transwell chambers. As shown schematically in (A), monolayers were treated with 100 nM OA-NO2 or DMSO (Veh) along with (B) PBS or (C) LPS (100 ng/ml) and incubated for 6 h. After 6 h neutrophils (4×105) were added to each upper chamber, and a chemoattractant mixture of LPS (100 ng/ml) plus TNF-α (25 ng/ml) in RPMI 1640 was added to each lower chamber. The chambers were incubated at 37°C for 1.5 h, and neutrophils that had transmigrated into the lower chambers were collected and counted. Data are representative of 3 independent experiments; *P < 0.05; **P < 0.01; ***P < 0.001.