Fig. 5.

metHb-induced apoptosis. (a) isolated neutrophils, (b) WBC. The apoptosis was assessed by detecting Annexin V⁺/7AAD⁺ cells. The % of live cells was calculated with Annexin⁻/7AAD⁻ cell population. Mock was buffer only treatment. Data are means of duplicate experiments. §P < 0.05, dose dependency by regression analysis. *, ¶ P < 0.05 by two-tailed Student's t-test.