Fig. 3.

Six1^−/−;Six4^−/− or Eya1^−/− embryos show defect in mesonephric tubules. (A,C,E) Lateral view and (B,D,F) sections through metanephric region of whole-mount embryos shown in A,C and E (plane of sections indicated by dashed lines in wild-type and mutant embryos respectively) stained with Pax2 riboprobe showing its expression in nephric duct, mesonephric tubules and metanephric progenitors in wild-type embryos (A,B). In the mutants, Pax2 expression in the nephric duct appears unaltered, whereas its expression in the caudal metanephric mesenchymal progenitors is absent (C-F). Moreover, only 3-4 pairs of mesonephric tubules (ms) at the rostral end were present in the Six1;Six4 (C) or Eya1 (E) mutant embryos.