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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Dev Dyn. 2015 Jun 18;244(7):866–873. doi: 10.1002/dvdy.24282

Fig. 4.

Fig. 4

Increased cell death and Osr1 expression in nephrogenic cord mesenchyme in Six1−/−;Six4−/− or Eya1−/− embryos. (A-C) TUNEL assay on sections of E9.75 embryos through caudal nephrogenic region as indicated by dashed lines in D-F. Numerous apoptotic cells (green) were detected in the intermediate mesoderm in the mutants. Apoptotic cells (means ± SD) were counted from 15 sections at 8 µm for each side (from 4-6 embryos for each genotype). P-values (between 0.01 to 0.087) were calculated using StatView t-test. (D-I) Lateral views of whole-mount wild-type (D,G), Six1−/−;Six4−/− double (E,H) or Eya1−/− single (F,I) mutant embryos stained with Osr1 riboprobe at E9.5 (D-F) and E10.0 (G-I). (J-L) Sections whole-mount stained embryos shown in D-F through mesonephric region as indicated by dashed lines. Osr1+ nephrogenic progenitors were detectable in the Six1;Six4 double or Eya1 single mutants at these earlier stages. Abb., nd, nephric duct.