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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Exp Cell Res. 2015 May 18;335(2):224–237. doi: 10.1016/j.yexcr.2015.05.008

Figure 1. PP6-C, SAPS1, SAPS2, and SAPS3 mRNA and protein content in control and PP6KD cells.

Figure 1

A. Cell lysates were prepared from control transduced cells and five cell lines that were transduced with shRNA targeting PP6-C. Western immunoblotting performed on triplicate samples was used to calculate the mean and standard deviation for the reduction in PP6-C protein content. B. Triplicate total RNA samples from control transduced and the 18.5 and 19.5 PP6KD HepG2 cell lines were analyzed by RT-PCR for PP6-C, the three SAPS and 18s ribosomal RNA. The resulting ethidium bromide-stained gels are shown. C. The results for PP6-C and 18s were quantified by densitometry and expressed as the ratio of PP6-C to 18s (mean and standard deviation of the triplicate analyses). D. Triplicate lysates from control transduced and the 18.5 and 19.5 PP6KD cell lines were analyzed by immunoblotting for PP6-C and the three SAPS. E. The gels shown in Panel D were analyzed by densitometry. Results were normalized to the mean intensity for the triplicate controls and expressed as the mean and standard deviation. Black bars, control cells; white bars, 18.5; gray bars, 19.5.