Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Genet. 2015 Jun 1;47(7):776–783. doi: 10.1038/ng.3324

Figure 1. Cardiac-specific Trbp knockout resulted in contraction defects in the heart.

Figure 1

a) qRT-PCR of Trbp mRNA levels in the hearts of postnatal day 2.5 cTNT-CreTG;Trbpfl/fl (TrbpcKO) and control littermate (Ctrl) mice. n = 3.

b) Trbp protein levels in the hearts of postnatal day 2.5 TrbpcKO and control mice were assayed with Western blot. β-tubulin serves as a loading control.

c) Kaplan-Meier survival curves of TrbpcKO and control mice. P<0.01.

d) Gross morphology and histology of TrbpcKO and control hearts at different time points. p2.5: postnatal day 2.5; 2w: 2 weeks after birth; 3w: 3 weeks after birth; 1m: 1 month after birth; 2m: 2 month after birth. Bars = 1.0 mm.

e) Fast green and Sirius red staining of TrbpcKO and control hearts from 2-month old mice. The portion of the heart represented in high magnification is boxed. Bar = 1.0 mm (upper panel); Bar = 500 µm (lower panel).

f) Echocardiography of left ventricle internal dimension at systolic (LVID;s) in TrbpcKO and control mice at indicated time points. n = 3–6.

g) Fractional shortening (FS%) of TrbpcKO and control mice at indicated time points. n = 3–6.

h) Anf mRNA level in the hearts of TrbpcKO and control mice at indicated time points. n = 3. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.