a) qRT-PCR of Myh6 and Myh7 mRNA levels in TrbpcKO and control hearts at 2 weeks (n = 3) and 1 month (n = 6).
b) Hierarchical clustering heatmap of 351 upregulated and 395 downregulated transcripts from 2-week old TrbpcKO and control hearts. Multiple fast-twitch (red) and slow-twitch (blue) myofiber genes and Hopx, Sox6 genes are marked.
c) The top 5 functional categories from Gene Ontology (GO, Molecular Function) analyses of dysregulated genes in TrbpcKO hearts.
d) The top 5 categories from KEGG pathway analysis of dysregulated genes in TrbpcKO hearts.
e) qRT-PCR of fast-twitch myofiber genes in 2-week old TrbpcKO hearts. n = 3.
f) qRT-PCR of slow-twitch myofiber genes in 2-week old TrbpcKO hearts. n = 3. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.