a) Schematic of AAV9 constructs and the experimental procedure.
b) Western blot to detect Trbp protein expression in 1-month old hearts. Gapdh serves as a loading control.
c) Kaplan-Meier survival curves of AAV-Trbp and AAV-Luc control injected TrbpcKO and control mice (TrbpcKO /AAV-Luc VS. TrbpcKO /AAV-Trbp, P<0.01).
d) Gross morphology and histology of heart samples from 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. Bars = 1.0 mm.
e) M-mode echocardiography images of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control.
f) Left ventricle internal dimension at systolic (LVID;s) and fractional shortening (FS%) of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control (n = 5–14).
g) Anf mRNA level in the hearts of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3.
h) qRT-PCR of fast-twitch myofiber genes in 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3.
i) qRT-PCR of slow-twitch myofiber genes in 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.