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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Genet. 2015 Jun 1;47(7):776–783. doi: 10.1038/ng.3324

Figure 3. Re-introduction of Trbp in TrbpcKO hearts rescued cardiac defects.

Figure 3

a) Schematic of AAV9 constructs and the experimental procedure.

b) Western blot to detect Trbp protein expression in 1-month old hearts. Gapdh serves as a loading control.

c) Kaplan-Meier survival curves of AAV-Trbp and AAV-Luc control injected TrbpcKO and control mice (TrbpcKO /AAV-Luc VS. TrbpcKO /AAV-Trbp, P<0.01).

d) Gross morphology and histology of heart samples from 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. Bars = 1.0 mm.

e) M-mode echocardiography images of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control.

f) Left ventricle internal dimension at systolic (LVID;s) and fractional shortening (FS%) of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control (n = 5–14).

g) Anf mRNA level in the hearts of 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3.

h) qRT-PCR of fast-twitch myofiber genes in 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3.

i) qRT-PCR of slow-twitch myofiber genes in 1-month old TrbpcKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.