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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Genet. 2015 Jun 1;47(7):776–783. doi: 10.1038/ng.3324

Figure 4. The function of Trbp is mediated by Sox6 in the heart.

Figure 4

a) Expression of Sox6 (left panel) and Trbp (right panel) in the hearts of 1-month old TrbpcKO and control mice injected with AAV-Scramble or AAV-Sox6 shRNA. n = 3.

b) Kaplan-Meier survival curves (TrbpcKO /AAV-Scramble VS. TrbpcKO /AAV-Sox6 shRNA, p<0.01).

c) Gross morphology and histology of the heart samples from 1-month old mice. Bars = 1.0 mm.

d) Left ventricle internal dimension at systolic (LVID;s) and fractional shortening (FS%) of 1-month old mice. n = 6–12.

e) Expression of Anf in the hearts of 1-month old mice. n = 3.

f) Expression of fast-twitch myofiber genes in the hearts of 1-month old mice. n = 3.

g) Expression of slow-twitch myofiber genes in the hearts of 1-month old mice. n = 3.

h) Schematic of AAV9 constructs (upper panel). Western blot to detect Flag-tagged Trbp protein expression in 1-week old hearts after AAV injection (lower panel). β-tubulin serves as a loading control.

i) Kaplan-Meier survival curves of AAV-Sox6 and AAV-Luc control injected mice (p<0.01).

j) Left ventricle internal dimension at systolic (LVID;s) and fractional shortening (FS%) of 1-month old mice injected with AAV-Sox6 or AAV-Luc control. n = 6–7.

k) Expression of fast-twitch myofiber genes in the hearts of 1-month old mice injected with AAV-Sox6 or AAV-Luc control. n = 3.

l) Expression of slow-twitch myofiber genes in the hearts of 1-month old mice injected with AAV-Sox6 or AAV-Luc control. n = 3. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.