Figure 5. Genome-wide identification of dysregulated miRNA species in Trbp^cKO hearts.

a) Scatter plot of the expression of 594 miRNAs between p2.5 Trbp^cKO and control hearts. 60 dysregulated miRNA species (derived from 53 miRNA genes) are marked red.

b) Hierarchical clustering heatmap of the 53 dysregulated miRNA genes between Trbp^cKO (n = 3) and control (n = 3) hearts. Significantly down-regulated miR-208a cluster is marked.

c) qRT-PCR of indicated miRNAs in p2.5 Trbp^cKO and control hearts. n = 3.

d) Northern blot of indicated miRNAs in p2.5 Trbp^cKO and control hearts. U6 serves as a loading control.

e) Quantification of pre-miR-208a (left panel) and mature miR-208a (right panel) from Northern blots of p2.5 Trbp^cKO and control hearts. n = 3.

f) qRT-PCR of miR-208a-3p and miR-499-5p in p2.5 Trbp^cKO and control hearts. n = 3.

g) qRT-PCR of miR-208a-3p, miR-378a-5p and miR-499-5p in the hearts of 1-month old Trbp^cKO and control mice injected with AAV-Trbp or AAV-Luc control. n = 3.

h) Northern blot of miR-208a-3p in the hearts of 1-month old Trbp^cKO and control mice injected with AAV-Trbp or AAV-Luc control. Both the precursor (pre-miR-208a) and mature product (miR-208a-3p) are shown. U6 serves as a loading control.

i) Northern blot of miR-208-3p in the hearts of 1-month old Trbp^cKO and control mice injected with AAV-Scramble or AAV-Sox6 shRNA. Both the precursor (pre-miR-208a) and mature product (miR-208a-3p) are shown. U6 serves as a loading control. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.