Figure 6. miR-208a is a Trbp target to mediate its function in TrbpcKO hearts.
a) Northern blot of miR-208a and miR-378a in the hearts of indicated mice. U6 serves as a loading control.
b) qRT-PCR of miR-208a in the hearts of indicated mice. n = 3.
c) Kaplan-Meier survival curves (TrbpcKOVS. TrbpcKO; miR-208aTG, P<0.01).
d) Gross morphology and histology of the heart samples from 1-month old mice. Bars = 1.0 mm.
e) Left ventricle internal dimension at systolic (LVID;s) and fractional shortening (FS%) of 1-month old mice. n = 7–9.
f) Anf mRNA level in the hearts of 1-month old mice with indicated genotypes. n = 3.
g) Expression of Sox6 in the hearts of 1-month old mice. n = 3.
h) Expression of fast-twitch myofiber genes in the hearts of 1-month old mice. n = 3.
i) Expression of slow-twitch myofiber genes in the hearts of 1-month old mice. n = 3.
j) Expression of miR-499-5p, miR-155-5p and miR-301a-3p in the hearts of 1-month old mice. n = 3.
k) Luciferase reporters were co-transfected with pcDNA-miR-208a or control and relative luciferase activity determined. n = 3.
l) Expression of Sox6 in the hearts of 2-week old mice. n = 3.
m) Expression of fast-twitch myofiber genes in the hearts of 2-week old mice. n = 3.
n) Expression of slow-twitch myofiber genes in the hearts of 2-week old mice. n = 3.
o) A working model signifying the Trbp:miR-208a:Sox6 linear pathway in the regulation of slow-twitch myofiber gene expression and cardiomyopathy. Values are expressed as the mean ± SD. NS: not significant, *: P<0.05, **: P<0.01.